Hif1a Knockout cell line(Hepa1-6)

Hif1a Knockout cell line(Hepa1-6) Order

Catalog#

YKO-M100

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Hif1a Knockout cell line (Hepa1-6)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Mouse

Gene Name

Hif1a

Gene ID

15251

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Target region

Knockout region: Exon 5 (based on transcript Hif1a-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Hepa1-6			STR Info (Cell bank) Cell Line:Hepa 1-6
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
4-2	229 【18.3】	233.12 【19.3】		18.3	19.3
5-5	347.54 【17】			17.0
6-4	299.48 【18】	303.51 【18】		18.0	19.0
6-7	345.94 【15】			15.0
9-2	220.65 【15】			15.0
12-1	229.12 【17】			17.0
15-3	176.95 【17】	181.02 【18】	185.08 【19】	17.0	18.0	19.0
18-3	151.1 【16】	155.50 【17】		16.0	17.0
X-1	399.97 【25】			25.0
D4S2408

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Hif1a gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hepa1-6 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Hif1a gene knockout Hif1a Knockout cell line (Hepa1-6) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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